Originating from Tuscan churches, the paintings displayed in this room are datable between the first half of the twelfth century and the beginning of the fourteenth. The technique is tempera on panel, on gold ground.

This large room is dominated by the three cusped panel paintings by Duccio di Boninsegna, Cimabue and Giotto; they all portray the Madonna Enthroned, surrounded by angels and saints, traditionally referred to as the Maestà.

These works are fundamental to an understanding of the styles of Tuscan painting between the thirteenth and the fourteenth century, and of the profound renewal which began in this period.

Cimabue's painting, while preserving many of the formal traits of the Byzantine style, is innovative in terms of the plastic strength. Duccio's Madonna expresses an exquisite synthesis between the more classical tendencies of the Eastern tradition and Gothic art.

Giotto's Maestà with its distinct representation of space, the focus on the light that models the figures and its naturalistic realism, marks a crucial turning-point in the evolution of Italian art.

Also present in the room are two Painted Crucifixes, a typically Italian church decoration, a dossal by Meliore, a diptych of the school of Bonaventura Berlinghieri and the Polyptych painted by Giotto for the Badia Fiorentina.