Entering the room, the visitor immediately sees opposite three works which are fundamental to an understanding of the early work and development of Leonardo da Vinci.

The Baptism of Christ was largely executed by Verrocchio, in whose workshop Leonardo received his early artistic training; the intervention of the young pupil is restricted to the head of an angel, the repainting of the landscape, and possibly the modelling of the body of Christ.
The Annunciation is recognised as an early work by Leonardo, already marked by a painting attentive to the atmospheric vibrations which the artist was to develop in later works.

The Adoration of the Magi was left unfinished, but is a work of striking conceptual and formal complexity.

Alongside Leonardo's works are important altarpieces by three masters active between the end of the fifteenth century and the beginning of the sixteenth: Perugino, Luca Signorelli and Lorenzo di Credi.